In the footage, Rakeyia Scott tells husband Keith Scott to get out of his car as Charlotte police surround him.
The clip does not show the actual shooting, or make clear if Mr Scott was carrying a gun, as police say.
Officers can be heard urging him to “drop the gun” but his wife is heard telling them he is unarmed.
On Friday evening, a few hundred protestors took to the streets, but the demonstrations was smaller than on the previous three nights.
They chanted “No justice, no peace” and “Release the video” – a call for the police to release their dash-cam and body-cam images of the incident.
The state governor has declared a state of emergency in the city, and a midnight curfew has been imposed for a second night running, after rioters looted businesses and threw objects at police on Tuesday and Wednesday nights.